Composite

Part:BBa_K4728020:Design

Designed by: Bryant Han   Group: iGEM23_Queens-Canada   (2023-10-10)


TPA transporter expression (TpaK)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1878
    Illegal PstI site found at 1984
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1878
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1984
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1878
    Illegal BglII site found at 640
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1878
    Illegal PstI site found at 1984
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1878
    Illegal PstI site found at 1984
    Illegal AgeI site found at 412
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All basic parts are golden gate cloning compatible (RFC1000), and the gene fragment containing tpaK included an RBS on both 5' and 3' end, with the fragment having MoClo overhangs TACT and AATG to allow for multicistronic transcriptional unit assembly.


Source

TpaK comes from the genome of Rhodococcus jostii and TphR comes from the genome of Comamonas testosteroni. All other parts originate from the 2023 iGEM distribution kit.

References